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Prof T.J. Britz - Researcher at Dept.
Food Science, US
Dr Gunnar Sigge - Researcher at Dept.
Food Science, US
Alison Ackerman - MSc student at Dept.
Food Science, US
Maryke Lötter - MSc student at
Dept. Food Science, US
Nicola Huisamen - MSc student at Dept.
Food Science, US
Amanda Brand - MSc student at Dept.
Food Science, US
Schalk van der Westhuizen - student/researcher
at Dept. Food Science, US
Ashley Hendricks - student/researcher
at Dept. Food Science, US
Corné Lamprecht - PhD student
at Dept. Food Science, US
Dr C.A. Barnardt - Enviro Services
Dr R. Strydom - Oz Purification
Fltr: Prof TJ BRitz, Nicola Huisamen, Amanda Brand,
Armelle Nstame Affane, Dr Gunnar Sigge,
Nadia Niemann, Schalk van der Westhuizen, Tshepo Kikine

Prototype 20 000 L UASB reactor |
Anaerobic Digestion
Various projects have been undertaken, investigating
aspec ts of anaerobic digestion of food and beverage
processing industry wastewaters mostly by upflow anaerobic
sludge blanket (UASB) technology.
The applicability of the process and optimization thereof
are the main objectives, although some troubleshooting
is also carried out for full-scale systems. Wastewaters
recently studied include: Fruit canning, winery, distillery,
fruit juice and gelatin effluents.

Effect of ozone on
food processing wastewater |
Advanced Oxidation Processes
Advanced oxidation processes (AOP's) are used to treat
various wastewaters, especially those containing difficult
to degrade compounds. Ozone, hydrogen peroxide, UV light
and certain catalys ts are the main methods that have
been used.
Ozone is especially useful and efficient and breaks
down to environmentally friendly oxygen after use.
These processes are often used in combination with biological
processes as pre- and post-treatments.

UASB granules |
Granulation
Formation of granules in the UASB process is a serious
rate-limiting process. The production of granules in
batch systems and the enhancement of the granulation
process are thus important areas of research.
Work is also done, using molecular techniques like
PCR and DGGE to classify and group organisms present
in granules and to follow the incorporation of potentially
useful organisms into "tailor-made" granules.
Corné van Schalkwyk
Mass culturing of granules for use in upflow
anaerobic sludge blanket (UASB) bioreactors by process
induction and microbial stimulation.
Marijké Lötter
Impact of polluted irrigation water on agricultural
products - pre- and post-harvest decay rates of contaminating
organisms.
Study Leader: Prof. T.J. Britz (University of Stellenbosch).
Co-Study Leader: Dr G.O. Sigge (University of Stellenbosch).
Alison Ackerman
Microbial investigation into the link between irrigation
water quality and safety of raw and minimally processesd
agricultural products.
Study Leader: Prof. T.J. Britz (University of Stellenbosch).
Co-Study Leader: Dr G.O. Sigge (University of Stellenbosch).
Amanda Brand
Evaluation of the growth profiles of surrogate coliforms
on minimally processed foods that had been spray-irrigated.
Study Leader: Prof. T.J. Britz (Stellenbosch University),
Co-Study Leader: Dr. G.O. Sigge (Stellenbosch University).
Nicola Huisamen
Impact of polluted irrigation water on the safety factor
of food products that are consumed raw.
Study Leader: Dr. G.O. Sigge (Stellenbosch University),
Co-Study Leader: Prof. T.J. Britz (Stellenbosch University).
Schalk van der Westhuizen
Enhancement of the methanogenic activity in a UASB treating
grain distillery wastewater.
Study Leader: Dr. G.O. Sigge (Stellenbosch University),
Co-Study Leader: Prof. T.J. Britz (Stellenbosch University).
Nadia Niemann
Bacterial source tracing from irrigation water to fresh
produce.
Study Leader: Prof. T.J. Britz (Stellenbosch University),
Co-Study Leader: Dr. G.O. Sigge (Stellenbosch University).
Tshepo Kikine
Survival of index organisms during irrigation of vegetables.
Study Leader: Prof. T.J. Britz (Stellenbosch University),
Co-Study Leader: Dr. G.O. Sigge (Stellenbosch University).
Ashley Henricks
Enhancement of the methane yield and bio-energy recovery
from a UASB treating Grain Distillery Wastewater (GDWW).
Study Leader: Dr. G.O. Sigge (Stellenbosch University),
Co-Study Leader: Prof. T.J. Britz (Stellenbosch University).
Publications:
BRITZ, T.J., TRNOVEC, W., VAN SCHALKWYK, C. & ROOS,
P. Enhanced granulation in upflow anaerobic sludge-bed
digesters (UASB) by process induction and microbial
stimulation. WRC, 1999, 116pp.
BRITZ, T.J., TRNOVEC W., & FOURIE P.C. Influence
of retention times and influent pH on the performance
of an upflow anaerobic sludge bioreactor treating cannery
waste waters. International Journal of Food Science
and Technology 2000; 35(1): 1-8.
SIGGE, G.O. 2000. Letting nature do the dirty
work. South African Food and Beverage Manufacturing
Review, 27(11), 32-33.
SIGGE, G.O., BRITZ, T.J., FOURIE, P.C., BARNARDT, C.
& STRYDOM, R. 2001. Use of ozone and hydrogen
peroxide in the post-treatment of UASB treated alkaline
fruit cannery effluent. Water Science and Technology,
44(5), 69-74.
STRYDOM, J.P., MOSTERT, J.F. & BRITZ, T.J. 2001.
Anaerobic digestion of dairy factory effluen ts . WRC
Report No 455/1/01. ISBN 1868457249. Published by the
Water Research Commission, Pretoria, South Africa.
SIGGE, G.O., BRITZ, T.J., FOURIE, P.C., BARNARDT, C.
& STRYDOM, R. 2002. Combining UASB technology
and advanced oxidation processes (AOP's) to treat food
processing wastewaters, Water Science and Technology,
45(10), 329-334.
SIGGE, G.O., BRITZ, T.J., FOURIE, P.C. & BARNARDT,
C. 2005. The efficacy of ozone as a pre- and post-treatment
option for UASB-treated food processing wastewaters,
Water Science and Technology, 52(1&2), 167-173.
SIGGE, G.O. & BRITZ, T.J. 2007. UASB Treatment
of a highly alkaline fruit cannery lye-peeling wastewater.
Water SA, 33(2), 275-278.
SIGGE, G.O., GREEN, J., DU PLESSIS, K.R. & BRITZ,
T.J. 2007. Investigating the effect of ozone on the
biodegradability of distillery wastewater. South African
Journal of Enology and Viticulture, 28(2), 155-162.
SIGGE, G.O., GIE, L-M. & BRITZ, T.J. 2008. Improving
the biodegradability of grain distillery wastewater
for use as UASB substrate by combinations of ozonation/enzymatic
pre-treatments. Water Science and Technology, 57(4),
547-552.
Presentations 2001-2008:
BRITZ, T.J., RONQUEST, L-C., KEYSER, M. & WITTHUHN,
R.C. Tailor-made UASB granules used for the treatment
of winery effluent, Cape Biotech 2001, Cape Town.
BRITZ, T.J., RONQUEST, L-C. & WITTHUHN, R.C. UASB
treatment of winery effluent with tailor-made granular
sludge. IWA World Congress on Anaerobic Digestion 2001,
Antwerpen, Belgium.
SIGGE, G.O., BRITZ, T.J., FOURIE, P.C., BARNARDT, C.
& STRYDOM, R. Combining UASB technology and advanced
oxidation processes (AOP's) to treat food processing
wastewaters, IWA World Congress on Anaerobic Digestion
2001, Antwerpen, Belgium.
BRITZ TJ, SIGGE GO, & VAN SCHALKWYK N. Use of ozone
as a pre-treatment to eliminate pectin gelling from
fruit processing wastewaters before anaerobic digestion.
10th Anaerobic Digestion Congress, Montreal, Canada
2004.
ELS ER, VAN ZYL PJ, & BRITZ TJ. Bioreactor for
mass culturing of UASB granules in twenty days. 10th
Anaerobic Digestion Congress, Montreal, Canada 2004.
KEYSER M, WITTHUHN RC, & BRITZ TJ. PCR-based DGGE
fingerprinting and identification of 'Eubacteria' present
in four different UASB granules. Anaerobic Digestion
2004, Montreal, Canada 2004.
SIGGE GO, BRITZ TJ, FOURIE PC, & BARNARDT CA. The
efficacy of ozone as a pre- and post-treatment option
for UASB treated food processing wastewaters. 10th World
Congress on Anaerobic Digestion, Montreal, Canada 2004.
KEYSER M, WITTHUHN RC, & BRITZ TJ. Fingerprinting
of the 'Eubacteria' in different UASB granules using
PCR-based DGGE. 13th Biennial Congress of South African
Society for Microbiology, Stellenbosch, South Africa
2004.
GREEN, J., SIGGE, G.O. & BRITZ, T.J. 2005. The
impact of ozonation on wetland systems for cellar effluent
degradation. South African Association for Food Science
and Technology Eigteenth Biennial Congress, Stellenbosch.
4 - 8 September 2005.
GIE, L-M., SIGGE, G.O. & BRITZ, T.J. 2005. Effect
of grain distillery wastewater on the efficiency of
UASB granules. South African Association for Food Science
and Technology Eighteenth Biennial Congress, Stellenbosch.
4 - 8 September 2005.
BRITZ, T.J., SIGGE, G.O. & VAN SCHALKWYK, N. 2005.
Impact Of Ozone Treatment On Pectin Gelling Of Apple
Juice Processing Wastewater. South African Association
for Food Science and Technology Eigteenth Biennial Congress,
Stellenbosch. 4 - 8 September 2005.
ACKERMANN, A., SIGGE, G.O. & BRITZ, T.J. 2007. Impact
of irrigating minimally-processed foods with river water
containing a high potentially-pathogenic load: A review.
South African Association for Food Science and Technology
Nineteenth Biennial Congress, Durban. 2 - 5 September
2007.
NTSAME AFFANE, A.L., SIGGE, G.O., KIDD, M. & BRITZ,
T.J. 2007. Demonstration of kefir grains microbial stability
using metabolic profiles as indicators. South African
Association for Food Science and Technology Nineteenth
Biennial Congress, Durban. 2 - 5 September 2007.
IIPUMBU, L.K., SIGGE G.O. & BRITZ, T.J. 2007. Proximate
composition of South African grown carob (Ceratonia
silliqua). South African Association for Food Science
and Technology Nineteenth Biennial Congress, Durban.
2 - 5 September 2007.
LÖTTER, M., SIGGE, G.O. & BRITZ, T.J. 2007.
Evaluation of the possible link between polluted irrigation
water and microbially contaminated minimally processed
foods in the Western Cape. South African Association
for Food Science and Technology Nineteenth Biennial
Congress, Durban. 2 - 5 September 2007.
BRITZ, T.J. & G.O. SIGGE. 2007. Impact and trends
on food-borne infections. Keynote Speaker, Environmental
Workshop, 2nd FIDSSA Congress, Cape Town. October 2007.
BRITZ, T.J., G.O. SIGGE., A. ACKERMANN & M. LÖTTER.
2007. Microbiological quality and safety scenarios as
applied to raw and minimally processed food products.
2nd ICFMH Workshop: Food Safety in Africa. International
Union of Microbiological Societies. Stellenbosch, 24-30
November 2007.
BRITZ, T.J., G.O. SIGGE, A. ACKERMANN & M. LÖTTER.
2007. Sustainability of irrigating food crops in areas
with a high incidence of river pollution. CHEMRAWN XII
conference. The Role of Chemistry in Sustainable Agriculture
and Human Well-being in Africa. Stellenbosch, 2-5 December
2007.
SIGGE, G.O., L. IIPUMBU, C. MULLER & T.J. BRITZ.
2008. Carob – food product value-adding opportunities.
IPUF 2008 – Indigenous Plant Use Forum. Graaff-Reinet,
7-10 July 2008.
SIGGE, G.O., BRITZ, T.J., FOURIE, P.C., BARNARDT, C.
& STRYDOM, R. 2001. Combining UASB technology and
advanced oxidation processes (AOP’s) to treat
food processing wastewaters. 9th IWA World Congress
on Anaerobic Digestion 2001, Antwerpen, Belgium, 2 –
5 September 2001.
SIGGE, G.O., BRITZ, T.J., FOURIE, P.C. & BARNARDT,
C.A. 2003. Ozone as a pre- and post-treatment option
to anaerobic digestion of fruit processing wastewaters.
IWA Biofilm Symposium, Cape Town. 14 – 18 September
2003.
McLACHLAN, T., SIGGE, G.O. & BRITZ, T.J. 2003. UASB
technology and its combination with ozonation in the
treatment of cellar effluent. IWA Biofilm Symposium,
Cape Town. 14 – 18 September 2003.
VAN SCHALKWYK, N., SIGGE, G.O. & BRITZ, T.J. 2003.
The use of ozonation as a pre-treatment to eliminate
pectin from fruit processing wastewaters before anaerobic
digestion. IWA Biofilm Symposium, Cape Town. 14 –
18 September 2003.
SIGGE, G.O., BRITZ, T.J., FOURIE, P.C. & BARNARDT,
C.A. 2004. The efficacy of ozone as a pre- and post-treatment
option for UASB treated food processing wastewaters.
10th World Congress on Anaerobic Digestion, Montreal,
Canada. 30 August – 3 September 2004.
BRITZ, T.J., SIGGE, G.O., VAN SCHALKWYK, N. 2004 Use
of ozone as a pre-treatment to eliminate pectin gelling
from fruit processing wastewaters before anaerobic digestion.
10th World Congress on Anaerobic Digestion, Montreal,
Canada. 30 August – 3 September 2004.
SIGGE, G.O., BRITZ, T.J. & BARNARDT, C.A. 2006.
Wastewater in the food industry – combining technologies
to facilitate treatment efficiency. EnviroWater –
International Conference and Exhibition on Water in
the Environment, Stellenbosch, 20 – 22 February
2006.
SIGGE, G.O., GIE, L-M. & BRITZ, T.J. 2007. Improving
the biodegradability of grain distillery wastewater
for use as UASB substrate by combinations of ozonation/enzymatic
pre-treatments. 11th World Congress on Anaerobic Digestion,
Brisbane, Australia. 23 – 27 September 2007.
SIGGE, G.O., BRITZ, T.J. & BARNARDT, C.A. 2008.
Combining technologies to facilitate treatment efficiency
of food industry wastewaters. IFT Annual Meeting &
Expo, New Orleans, USA, 29 June – 2 July 2008.
ACKERMANN, A., SIGGE, G.O. & BRITZ, T.J. 2008. Survival
of potential pathogens (Escherichia coli) on green beans,
peas and cocktail tomatoes treated with polluted irrigation
water. Food Micro, Aberdeen, Scotland, 2008.
SIGGE, G.O., BARNARDT, C.A. & BRITZ, T.J. 2008.
Effective wastewater treatment for small-scale food
and beverage processors: The winery experience.14th
IUFoST World Congress of Food Science and Technology
“Food for Health and Wellbeing”, Shanghai,
China, 19-23 October 2008.
Patents :
BRITZ, T.J. Method of microbial granule formation.
SA Patent No. 99/1877, 1999
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