James Lloyd

Dr James Lloyd

I specialize in the study of carbon metabolism in plants. Prior to starting work at the IPB I had worked in the UK, Germany and Denmark mainly analyzing plants altered in starch metabolism. Starch is a very important product produced by plants which is used in industry, so my research had a direct industrial application and led to the publication of many high profile papers. Since I started in South Africa I have continued with this research area, and am currently developing novel methods of virus induced gene silencing in order to study it further. In addition I am developing methods to analyze many metabolites simultaneously in plant extracts, which should enable us to come to a better understanding of how plant metabolism works.

List of Publications

  • George GM, Bauer R, Blennow A, Kossmann J, Lloyd JR (2012) Virus induced multiple gene silencing to study metabolic pathways in plants: Silencing the starch degradation pathway in Nicotiana benthamiana Biotechnology Journal in press
  • Lloyd JR/Lütgen H, Glaring MA, Baunsgaard L, Laursen KH, Haldrup A, Kossmann J, Blennow A. (2010) Repression of both isoforms of disproportionating enzyme leads to higher malto-oligosaccharide content and reduced growth in potato. Planta 232: 1127-1139
  • Kötting O, Kossmann J, Zeeman SC, Lloyd JR. (2010) Regulation of starch metabolism: the age of enlightenment? Current Opinions in Plant Biology 13: 1-9
  • George GM, van der Merwe MJ, Nunes-Nesi A, Bauer R, Fernie AR, Kossmann J, Lloyd JR. (2010) Virus-induced gene silencing of plastidial soluble inorganic pyrophosphatase impairs essential leaf anabolic pathways and reduces drought stress tolerance in Nicotiana benthamiana. Plant Physiology 154, 55-66
  • Ferreira SJ, Kossmann J, Lloyd JR, Groenewald J-H. (2008) The reduction of starch accumulation in transgenic sugar cane cell suspension culture lines. Biotechnology Journal 3, 1-9.
  • Lloyd JR, Kossmann J, Ritte G. (2005) Leaf starch degradation comes out of the shadows. Trends in Plant Science 10, 130-137
  • Schulze S, Mant A, Kossmann J, Lloyd JR. (2004) Identification of an Arabidopsis inorganic pyrophosphatase capable of being imported into chloroplasts. FEBS Letters 565, 101-105.
  • Lloyd JR, Blennow A, Burhenne K, Kossmann J. (2004) Repression of a novel isoform of disproportionating enzyme (stDPE2) in potato leads to inhibition of starch degradation in leaves, but not tubers stored at low temperature. Plant Physiology 134, 1347-1354
  • Obiadalla-Ali H, Fernie A, Lytovchenko A, Kossmann J, Lloyd JR. (2004) Inhibition of chloroplastic fructose 1,6-bisphosphatase in tomato fruits leads to surprisingly small changes in carbohydrate metabolism, but decreases fruit size. Planta 219, 533-540.
  • Obiadalla-Ali H, Fernie, A, Kossmann J, Lloyd JR. (2004) Developmental analysis of carbohydrate metabolism in tomato (Lycopersicum esculentum c.v. Micro-Tom) fruits. Physiologia Plantarum 120, 196-204
  • Ritte G, Steup M, Kossmann J, Lloyd JR. (2003) Analysis of starch phosphorylating activity in the sex1 mutant of Arabidopsis thaliana. Planta 216, 798-801
  • Westram A, Lloyd JR, Roessner U, Riesmeier J, Kossmann J (2002) Increases of 3-phosphoglyceric acid in potato plants through antisense reduction of cytoplasmic phosphoglycerate mutase impairs photosynthesis and growth, but does not increase starch contents. Plant, Cell & Environment. 25, 1133-1143
  • Ritte G, Lloyd JR, Eckermann N, Rottmann A, Kossmann J, Steup M. (2002) The starch related R1 protein is an -glucan dikinase. Proceedings of the National Academy of Sciences U.S.A. 99, 7166-7171.
  • Scheidig A, Frölich, A, Schulze, S, Lloyd, JR, Kossmann, J. (2002) Down-regulation of a chloroplast–targeted b-amylase that is capable of degrading potato starch granules leads to a starch-excess phenotype in leaves. The Plant Journal. 30, 581-591.
  • Yu T-S, Kofler H, Häusler RE, Hille D, Flügge U-I, Zeemann SC, Kossmann J, Lloyd JR, Ritte G, Steup M, Lue W-L, Chen J, Weber A. (2001) Sex1 is a general regulator of starch degradation in plants and not the chloroplast hexose transporter. The Plant Cell 13, 1907-1918.
  • Kossmann, J, Lloyd, JR. (2000) Understanding and influencing starch biochemistry. Critical Reviews in Plant Sciences 19, 171-226.
  • Lloyd JR, Springer F, Buléon A, Müller-Röber B, Willmitzer L, Kossmann J. (1999) The influence of alterations in ADP glucose pyrophosphorylase activities on starch structure and composition in potato tubers. Planta 209, 230-238.
  • Lloyd JR, Landschütze V, Kossmann J. (1999) Simultaneous antisense inhibition of two starch synthase isoforms in potato tubers leads to accumulation of grossly modified amylopectin. Biochemical Journal 338, 515-521.
  • Kossmann J, Abel GJW, Springer F, Lloyd JR, Willmitzer L. (1999) Cloning and functional analysis of a cDNA encoding a starch synthase from potato (Solanum tuberosum L.) that is predominantly expressed in leaf tissue. Planta, 208, 503-511.
  • Heyer, AG, Lloyd JR, Kossmann, J. (1999) Production of modified polymeric carbohydrates. Trends in Biotechnology 10, 169-174.
  • Craig, J,  Lloyd, JR, Tomlinson, K, Barber, L, Edwards, A, Wang, TL, Martin, C, Hedley, C, Smith, AM. (1998) Mutations in the gene encoding starch synthase II profoundly alter amylopectin structure in pea embryos. The Plant Cell  10, 413-426.
  • Tomlinson, KL; Lloyd JR; Smith AM. (1997) Importance of isoforms of starch-branching enzyme in determining the structure of starch in pea leaves.  The Plant Journal 11, 31-43.
  • Lloyd, JR; Bull, VJ; Hedley, CL; Ring, SG. (1996) Determination of the effects and interactions of the r and rb mutations on the structure of amylose and amylopectin in pea (Pisum sativum) Carbohydrate Polymers 47, 45-49.
  • Lloyd, JR; Wang, TL; Hedley, CL. (1996) An analysis of seed development in Pisum sativum XIX.  Effect of mutant alleles at the r and rb loci on starch grain size and on the content and composition of starch in developing pea seeds. Journal of Experimental Botany 47, 171-180.
  • Hedley, CL; Lloyd, JR; Ambrose, MJ; Wang, TL  (1994) An analysis of seed development in Pisum sativum XVII.  The effect of the rb locus alone and in combination with r on the growth and development of the seed.  Annals of Botany 74, 365-371.