Our Publications

2010

• Basson CE, Groenewald JH, Kossmann J, Cronjé C, Bauer R: Sugar and acid-related quality attributes and enzyme activities in strawberry fruits: invertase is the main sucrose hydrolysing enzyme. Food chemistry, 2010 121, 1156 – 1162
  
  
• Bastian R, Dawe A, Meier S, Ludidi N, Bajic VB, Gehring C: Gibberelic acid and cGMP-dependent transcriptional regulation in Arabidopsis Thaliana. Plant Signaling & Behavior, 2010 5, 1-9
  
  
• Keyster M, Klein A, Ludidi N: Endogenous NO levels regulate nodule functioning. Potential rorle of CGMP in nodule functioning? Plant Signaling & Behavior, 2010 5:12, 1-3
  
  
• Kiggundu A, Muchwezi J, van der Vyver C, Viljoen A, Vorster J, Schlueter U, Kunert K, Michaud D: Deleterious effects of plant cystatins against the banana weevil Cosmopolites sordidus. Archives of insect biochemistry and physiology, 2010 73, 87-105
  
  
• Koetting O, Kossmann J, Zeeman SC, Lloyd JR: Regulation of starch metabolism: the age of enlightenment? Current opinion in Plant Biology, 2010 13, 1-9
  
  
• Leach J, Keyster M, Du Plessis M, Ludidi N: Nitric oxide synthase activity is required for developemnt of functional nodules in soybean. Journal of Plant Physiology
  
  
• Lütken H, Lloyd JR, Mikkel AG, Baunsgaard L, Holst Laursen K, Haldrup A, Kossmann J, Blennow A: Repression of both isoforms of disproportionating enzyme leads to higher malto-oligosacchardie content and reduced growth in potato. Planta 2010 August
  
  
• Rossouw D, Kossmann J, Botha FC, Groenewald JH: Reduced neutral invertase activity in the culm tissues of transgenic sugarcane plants results in a decrease in respiration and sucrose cycling and an increase in the sucrose to hexose ration. Functional Plant Biology, 2010 37, 22-31
  
  
• Van der Merwe MJ, Groenewald JH, Stitt M, Kossmann J, Botha FC: Downregulation of pyrophosphate: d-fructose-6-phosphate 1-phosphotransferase activity in sugarcane culms enhances sucrose accumulation due to elevated hexose –phophate levels. Planta, 2010 231, 595-608
  
  
• Van der Vyver C: Genetic transformation of the euploid Saccharum officinarum via direct and indirect emryogenesis. Sugar Tech, 2010 12 (1) 21-25
  
  
• Van der Vyver C., Stander C., Kossmann J. And Groenewald H. Establishment of alternative selection systems for transgenic sugarcane callus. In: Aspects of Applied Biology 96, 2010, Agriculture: Africa’s “engine for growth” – Plant science & biotechnology hold the key, pp. 291-295.
  
  
• Zeeman SC, Kossmann J, Smith AM: Starch: its metabolism, evolution, and biotechnological modification in plants. Annual Review of Plant Biology, 2010 61, 1-15.26
  

2009

• Bauer R, du Toit M, Kossmann J: Influence of environmental parameters on production of the acrolein precursor 3-hydroxypropionaldehyde by Lactobacillus reuteri DSMZ 20016 and its accumulation by wine lactobacilli. International Journal of Food Microbiology, 2009.10.012
  
  
• Bauer R, Bekker J P, van Wyk N, du Toit C, Dicks L M T, Kossmann J: Exopolysaccharide production by lactose-hydrolyzing bacteria isolated from traditionally fermented milk. International Journal of Food Mircobiology, 2009.02.020
  
  
• Esterhuyse MM, Venter M, Veldhoen N, Helbing CC, van Wyk JH: Characterization of vtg-1 mRNA expression during ontogeny in Oreochromis mossambicus (PETERS). Journal of Steroid Biochemistry & Molecular Biology, 2009.07.001
  
  
• Nashilevitz S, Melamed-Bessudo C, Aharoni A, Kossmann J, Wolf S, Levy AA: The legwd mutant uncovers the role of starch phophorylation in pollen development and germination in tomato. The Plant journal, 2009 57, 1-13 (online 2008)
  

2008

• Bauer R, Nieuwoudt H, Bauer FF, Kossmann JM, Koch KR, Esbensen KH:FTIR spectroscopy for grape and wine analysis. 2008 Analytical Chemistry,2008 1371-1379
  
  
• Burger BV, Viviers MZ, Bekker JPI, le Roux M, Fourie WB, Weibchen G: Chemicals characterization of territorial marking fluid of male bengal tiger, Paanthera tigris. J Chem Ecol DOI 10.1007? s10886-008-9462
  
  
• Cullis CA, Voster BJ, Van der Yvver C., Kunert KJ. Transfer of genetic material between the chloroplast and nucleus: how is it related to stress in plants? Annals of Botany. : 1-9
  
  
• Ferreira SJ, Kossmann J, Lloyd JR, Groenewald JH: The reduction of starch accumulation in transgenic sugarcane cell suspension culture lines. Biotechnology journal, 2008 1398-1406
  
  
• Martins CM, Beyene G, Hofs JL, Krueger K, Van der Vyver C, Schlueter U, Kunert KJ: Effect of water-deficit stress on cotton plants expressing the Bacillus thuringiensis toxin. Annals of Applied Biology 2008 255-262
  
  
• Sun H, Scharff-Poulsen AM, Gu H, Jakobsen I, Kossmann JM, Frommer WB, Almdal K: Phosphate sensing by fluorescent reporter proteins embedded in polyacrylamide nanoparticles. ACSNano 2.1 19-24
  
  
• White EJ, Venter M, Hiten NF, Burger JT: Modified cetyltrimethlammonium bromide method improves robustness and versatility: the benchmark for plant RNA extraction/Biotechnology Journal, 2008 1424-1428
  
  
• Kleinert A, Venter M, Kossmann J, Valentine A J: Role of Pepc isoforms in Phosphate-deficient nodules. In: Biological Nitrogen Fixation. Towards Poverty Alleviation through Sustainable Agriculture. Springer Science. 2008
  

2007

• Groenewald J-H & Botha FC (2007). Down-regulation of pyrophosphate: fructose 6-phosphate 1-phosphotransferase (PFP) activity in sugarcane enhances sucrose accumulation in immature internodes. Transgenic Research (in press, DOI 10.1007/s11248-007-9079-x).
  
  
• Groenewald J-H & Botha FC (2007). Molecular and kinetic characterisation of sugarcane pyrophosphate: fructose-6-phosphate 1-phosphotransferase (PFP) and its possible role in the sucrose accumulation phenotype. Functional Plant Biology 34: 517-525.
  
  
• Ludidi NN, Pellny TK, Kiddle G, Dutilleul C, Groten K, VAN Heerden PD, Dutt S, Powers SJ, Romer P, Foyer CH. (2007) Genetic variation in pea (Pisum sativum L.) demonstrates the importance of root but not shoot C/N ratios in the control of plant morphology and reveals a unique  relationship between shoot length and nodulation intensity. Plant, Cell & Environment 30:1256-1268.
  
  
• Rossouw D, Bosch S, Kossmann JM, Botha FC & Groenewald J-H (2007). Down-regulation of neutral invertase activity in sugarcane cell suspension cultures leads to increased sucrose accumulation. Functional Plant Biology 34: 490-498.
  
  
• Venter, M (2007). Synthetic promoters: genetic control through cis engineering. Trends in Plant Science 12 (3): 118 -124   
  
  
• Taylor, NJ, PN Hills and J van Staden.  (2007).  Cell division versus cell elongation: the control of radicle elongation during thermoinhibition of Tagetes minuta achenes. Journal of Plant Physiology 164: 1612-1625
  

2006

• Venter M, Groenewald J-H & Botha FC (2006). Sequence analysis and transcriptional profiling of two vacuolar H+-pyrophosphatase isoforms in Vitis vinifera. Journal of Plant Research (2006) 119: 469–478 (DOI 10.1007/s10265-006-0009-4).
  

2005

• Botha FC (2005). Biotechnology continues to move closer to wide-scale application in the industry: the IVth Molecular Biology Workshop, held at CIRAD, Montpellier, France. Proc Int Soc Sug Cane Technol 25: 585-591.
  
  
• Gouws LM, Osmond BC, Schurr U, Walter A (2005) Distinctive diel growth cycles in leaves and cladodes of CAM plants: differences from C3 plants and putative interactions with substrate availability, turgor and cytoplasmic pH. Functional Plant Biology (32): 421–428
  
  
• Schäfer WE, Rohwer JM and Botha FC (2005). Partial purification and characterization of sucrose synthase in sugarcane. J Plant Phys 162: 11-20.
  
  
• Watt DA, McCormick AJ, Govender C, Carson DL, Cramer MD, Huckett BI and Botha FC (2005). Increasing the utility of genomics in unraveling sucrose accumulation. Field Crops Res 92: 149-158.
  

2004

• Bosch S, Grof CPL and Botha FC (2004). Expression of neutral invertase in sugarcane. Plant Sci 166: 1125-1133.
  
  
• Hoepfner SW and Botha FC (2004). Purification and characterization of fructokinase from the culm of sugarcane. Plant Sc 167: 646-654.
  
  
• Schäfer WE, Rohwer JM and Botha FC (2004). Protein-level expression and localization of sucrose synthase in the sugarcane culm. Physiologia Plantarum 121: 187-195.
  
  
• Schäfer WE, Rohwer JM and Botha FC (2004). A kinetic study of sugarcane sucrose synthase. Eur J Biochem 271: 3971-3977.
  

2003

• Bosch S, Rohwer JM and Botha FC (2003). The sugarcane metabolome. Proc S Afr Sug Technol Ass 77: 129-133.
  
  
• Hoepfner SW and Botha FC (2003). Expression of fructokinase isoforms in the sugarcane culm. Plant Physiology and Biochemistry 41: 741-747.
  
  
• Van der Merwe J, Groenewald S and Botha FC (2003). Isolation and evaluation of a developmentally regulated sugarcane promoter. Proc S Afr Sug Technol Ass 77: 146-149.
  
  
• Venter M and Botha FC (2003). Promoter analysis and transcription profiling: Integration of genetic data enhances understanding of gene expression. Physiologia Plantarum 119: 1-10
  
  

2002

• Bindon KA and Botha FC (2002). Carbon allocation to the insoluble fraction, respiration and triose-phosphate cycling in the sugarcane culm. Physiologia Plantarum 116: 12-19.
  
  
• Carson DL. and FC Botha (2002). Genes expressed in sugarcane maturing internodal tissue. Plant Cell Reports 20: 1075-1081.
  
  
• Carson DL, Huckett BI and Botha FC (2002). Sugarcane ESTs differentially expressed in immature and maturing internodal tissue. Plant Sci 162: 289-300.
  
  
• Carson DL, Huckett BI and Botha FC (2002). Differential gene expression in sugarcane leaf and internodal tissues of varying maturity. S A J Bot 68: 434
  
  
• Carson DL, Huckett BI and Botha FC (2002). Gene discovery and expression analysis in sugarcane – the story so far. Proc S Afr Sug Technol Ass 76: 224-227.
  
  
• Turner W and Botha FC (2002). Purification and kinetic properties of UDP-glucose dehydrogenase from sugarcane. Arch BiochemBiophys 407: 209-216.
  

2001

• Bindon KA and Botha FC (2001). Tissue disks as an experimental system for metabolic flux analysis in the sugarcane culm. S A J Botany 67: 244-249.
  
  
• Botha FC (2001). Book review on the history of plant biotechnology. Nature 414: 397-398.
  
  
• Botha FC and Rohwer J (2001). Kinetic modelling of sucrose metabolism: a powerful predictive tool for genetic manipulation of sugar content in sugarcane. Proc S Afr Sug Technol Ass 75: 99-100.
  
  
• Botha FC, Sawyer BJB and Birch RG (2001). Sucrose metabolism in the culm of transgenic sugarcane with reduced soluble acid invertase activity. Proc Int Soc Sug Cane Technol 24: 588-591.
  
  
• Carson DL, Huckett BI and Botha FC (2001). Genomics Research at SASEX: Perspectives from a small-scale programme. Proc Int Soc Sug Cane Technol 24: 539-541.
  
  
• Groenewald J-H and Botha FC (2001). Manipulating sucrose metabolism with a single enzyme: pyrophosphate-dependent phosphofructokinase (PFP). Proc S Afr Sug Technol Ass 75: 101-103.
  
  
• Groenewald J-H and Botha FC (2001). Down regulating pyrophosphate-dependent phosphofructokinase (PFP) in sugarcane. Proc Int Soc Sug Cane Technol 24: 592-594.
  
  
• Rohwer JM and Botha FC (2001). Analysis of sucrose accumulation in the sugarcane culm on the basis of in vitro kinetic data. Biochem J 358: 437-445.
  
  
• Snyman SJ, Huckett BI, Botha FC and Watt MP (2001). A comparison of direct and indirect somatic morphogenesis for the production of transgenic sugarcane (Saccharum spp. hybrids). Acta Hort 560: 105-108.
  

2000

• Bindon KA and Botha FC (2000). Tissue disks as an experimental system for metabolic flux analysis in the sugarcane culm. S A J Bot 66: 260-264.
  
  
• Botha FC and Black K.G. (2000) . Sucrose phosphate synthase and sucrose synthase activity during maturation of internodal tissue in sugarcane. Australian JPlant Physiol 27: 81-85.
  
  
• Carson DL and Botha FC (2000). Preliminary analysis of Expressed Sequence Tags for sugarcane. Crop Sci 40: 1769-1779.
  
  
• Carson DL, Huckett BI and Botha FC (2000). Patterns of gene expression in sugarcane monitored using cDNA macroarrays. Proceedings of the South African Sugar Technologists’ Association 74: 181-183
  
  
• Groenewald J-H, Hiten F and Botha FC (2000). The introduction of an inverted repeat to the 5’ untranslated leader sequence of a transgene strongly inhibits gene expression. Plant Cell Reports 19: 1098-1101.
  
  
• Meyer GM, Keeping MB, Watt DA, Cassim TN, Botha FC and Huckett BI (2000). A wild-type insecticidal gene from a bacterium is poorly expressed in sugarcane: an overview. Proceedings of the South African Sugar Technologists’ Association 74: 184-185
  
  
• Rose S and Botha FC (2000). Distribution patterns of neutral invertase and sugar content in sugarcane internodal tissues. Plant Physiol Biochem 38: 819-824.
  
  
• Snyman SJ, Watt MP, Huckett BI and Botha FC (2000) Direct somatic embryogenesis for rapid, cost effective production of transgenic sugarcane (Saccharum spp hybrids). Proceedings of the South African Sugar Technologists’ Association 74: 186-187.